Akademik Veri Yönetim Sistemi
Tezin Türü: Doktora
Tezin Yürütüldüğü Kurum: İstanbul Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Türkiye
Tezin Onay Tarihi: 2017
Tezin Dili: Türkçe
Öğrenci: Bilgin Candar
Danışman: Ercan Arıcan
Özet:
Drought is one of the adverse
environmental stress factor affecting plant development and production and
limiting crop yield. Plants exposed to drought stress have to re-establish
cellular homeostasis by altering gene expression programmes at the
transcriptional, post-transcriptional and post-translational levels. MicroRNAs
(miRNAs) are an important class of small regulatory RNAs, because they regulate
the expression of their target protein coding genes at post-transcriptional
level. Plant miRNAs play an active rol in growth, development, fruit ripening
and biotic and abiotic stress responses. Tomato (Solanum lycopersicum)
is one of the economically important field crop and moderatelly tolerant to
drougt stress. So drought stress has negative impact on tomato plants. However,
drought-responsive miRNAs in tomato cause yield loss has not been identified.
The goal of this thesis study is to identify induced or suppressed miRNAs and
target mRNAs under drought stress in two differently tolerant tomato plants.
Firstly, the two different drought tolerant (one is more tolerant and another
is more sensitive) tomato genotypes will expose to in vitro drought stress by using polyethylene
glycol (PEG). Total RNAs will be extracted from root samples and sent to
Beijing Genomics Institute (BGI) for small RNA and degradome sequencing.
According to high-throughput sequence data will be analyzed with bioinformatic
tools and drought-responsive miRNAs and target mRNAs will be identified. The
results are expected to contribute better understanding of miRNA-controlled
gene regulation during drought stress for plant adaptation and help in devising
strategies to improve crop plant tolerance under drought stress conditions,
using biotechnological approaches.