AMARANTH YAĞI İÇEREN TOPİKAL UYGULAMAYA YÖNELİK KOZMESÖTİK ETKİLİ FORMÜLASYONLARIN GELİŞTİRİLMESİ


Thesis Type: Postgraduate

Institution Of The Thesis: Istanbul University, Faculty Of Pharmacy , Department Of Pharmaceutical Technology, Turkey

Approval Date: 2023

Thesis Language: Turkish

Student: ABDULAZİZ KAŞİF

Supervisor: Ebru Altuntaş

Abstract:

Vegetable oils are known for their beneficial effects in the cosmetic industry as they have many biological activities.

Amaranth oil (Amaranthus cruentus oil) is a vegetable oil widely recognized for its health properties with its high squalene content (an important antioxidant and antimicrobial agent).

Amaranth oil is recognized as a bioactive natural product with abundant chemical composition in Ω-fatty acids, especially linoleic and oleic acids, phytosterols and tocopherols . The superior content in Ω-6 and 9 fatty acids leads to a beneficial rejuvenating effect on the skin, a softening and regenerating skin effect (wound healing effect) and increased hydration effect.

Squalene from amaranth oil significantly reduces free radical oxidative damage to the skin. Furthermore, phytosterols in amaranth oil fractions have been reported to anti-inflammatory and antimicrobial effects .

In this thesis, it is aimed to develop a cosmetic nanoemulgel formulation containing amaranth oil with proven efficacy and safety, which will support the improvement of skin wound and scar appearance. For this purpose, firstly, amaranth fixed oil was standardized in terms of fatty acid composition after derivatization processes and this standardization was demonstrated in detail by using Gas Chromatography method with different detectors (Diode array detector (DAD), Mass Spectrometer (KS) etc.).

Then, dermatological nanoemulgel formulations containing standardized Amaranth oil were prepared using the ultrasonic emulsification method. A preliminary evaluation was made in terms of kinetic and thermodynamic stability by applying accelerated stability tests, centrifuge and thermal tests to the prepared formulations. As a result of these tests, stable nanoemulgels were selected and their physicochemical stability was evaluated for 3 months. Finally, after stability tests, it was provided to demonstrated the potential effects of the use of the developed nanotechnological formulation on damaged skin by conducting in vitro cytotoxicity (MTT Test) and in vitro efficacy tests (antimicrobial activity and wound healing effect (scratch assay)) of the most suitable and optimized nanoemulgel formulation.