Çok duvarlı karbon nanotüplerden bakteriye özgü biyosensör geliştirilmesi


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BEKTAŞ M. (Executive), ÜSTÜNSOY R., ERTAŞ T., DİNÇ B., CEYLAN A. N.

Project Supported by Higher Education Institutions, 2021 - 2022

  • Project Type: Project Supported by Higher Education Institutions
  • Begin Date: April 2021
  • End Date: November 2022

Project Abstract

Detection of pathogenic bacteria has vital importance in clinical diagnosis, food industry, water quality and health problems. Techniques for detection and diagnosis of bacteria are originated from colony culture counting, polymerase chain reactions and immunology tests. In concern of food and environmental health, producing fast and precise devices in order to face with adverse effects of bacterial problems is important. Carbon nanotubes(CNT) are known with their superior physicochemical characteristics which made them very apropriate for building fast operative and precise biosensors. In recent years, CNT’s modified considering necessities, are being used in bacteria and virus detecting devices. Properties that made CNT’s being used in biosensor devices are being more resistive against physical stress than steel, bearing extensive binding site surface areas and having high electiric conductivity. Multi-walled CNTs’ (MWCNT) have high electron conductivity and surface area than singlewalled CNTs’ (SWCNT). MWCNTs that we use are 0.5-2 µm long, and they have ~8 nm outer diameter. These CNTs are functionalized with strong acids in order to construct carboxyl groups in their surface areas and then, they used as drug and protein cariers in previous reasearches. For characterization tecniques differential scannig calorimetry (DSC), TGA, FTIR and Raman spectroscopy, TEM and SEM are used. Information that is collected from these techniques and MWCNTs itselves will be used in the production of biosensor devices. Producing steps will include sonication of tungsten wire and MWCNTs with N-dimethylformamide in order to preserve sterilization of wires and dispersion of MWCNTs. Then, MWCNTs and wires will provide a complex by occuring surface modification followed by drying at 150 °C. Additionally, final wires will be fluxed by streptavidin and new complex will be constructed. Fort he measurements, picoampermeters will be used on MWCNT biosensons feeded by E.Coli (K12) bacteria. TEM images of MWCNT sensors will be taken and they will analyzed according to their precision. Precision measurements will be held before and after adding E.coli K12. Additionally, these experiments Yazım alanları gerektiği kadar uzatılabilir will be repeated with different concentrations of E.coli K12. By this way, detection of patogens will be more efficient with the help of molecularly modifacionable and high conductive MWCNTs that is complexed with tungsten microwire, without need of bacterial amplification from samples.