A Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases

Inci F., Ozen M. O., Saylan Y., Miansari M., Cimen D., Dhara R., ...Daha Fazla

ADVANCED SCIENCE, cilt.5, sa.9, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 9
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/advs.201800121
  • Dergi Adı: ADVANCED SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: bioinspired materials, differential extraction, DNA casework backlog, forensic cases, microfluidics, HUMAN CHORIONIC-GONADOTROPIN, EPITHELIAL-CELLS, MONOCLONAL-ANTIBODY, FTIR SPECTROSCOPY, EGG BINDING, RECOVERY, OLIGOSACCHARIDE, SPERMATOZOA, SEPARATION, DNA
  • İstanbul Üniversitesi Adresli: Evet

Özet

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis(X)), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epithelial fraction. Captured sperm are then lysed on-chip and sperm DNA is isolated. This method reduces assay-time from 8 h to 80 min, providing an inexpensive alternative to current differential extraction techniques, accelerating identification of suspects and advancing public safety.