The association of oxidative stress and DNA damage with XRCC1 and XRCC3 polymorphisms in radiology technicians


Soylemez E., Ozcagli E., Korkmaz S., Tok O. E., Aydin M. S., Omurtag G. Z.

TOXICOLOGY AND INDUSTRIAL HEALTH, vol.38, pp.70-79, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38
  • Publication Date: 2022
  • Doi Number: 10.1177/07482337211062680
  • Journal Name: TOXICOLOGY AND INDUSTRIAL HEALTH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, BIOSIS, Communication Abstracts, EMBASE, Environment Index, Index Islamicus, MEDLINE, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Page Numbers: pp.70-79
  • Keywords: Radiology technicians, DNA Damage, oxidative stress, XRCC1 (Arg399Gln), XRCC3 (Thr241Met), occupational exposure, COMET ASSAY, IONIZING-RADIATION, REPAIR GENES, OCCUPATIONAL-EXPOSURE, LUNG-CANCER, RISK, LYMPHOCYTES
  • Istanbul University Affiliated: Yes

Abstract

Ionizing radiation has widespread use in medicine in the diagnosis and treatment of many medical conditions. Radiology technicians are one group that is occupationally exposed to low doses of radiation. There are questions regarding whether low dose exposure to radiation could have long-term health consequences. Assessing the effect of radiation on genetic material is essential for appraising long-term health results. Hereditary variations in DNA repair genes cause differentiation in individual responses to radiation related health effects. This study aimed to determine oxidative stress and DNA damage, and their relationship to XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) polymorphisms in radiology technicians occupationally exposed to low dose radiation. Peripheral blood samples were collected from 45 radiology technicians and age-matched with 40 healthy control individuals working in office environments. Our results showed that radiology technicians had significantly greater oxidative stress and DNA damage than the control group, and women appeared more susceptible to occupational radiation exposure than men. Individuals with wild-type genotypes for XRCC1 (Arg/Arg) and XRCC3 (Thr/Thr) had less DNA damage. Lower DNA damage levels could be explained by the enhanced capacity to repair low dose radiation induced DNA damage. Further studies are needed to evaluate the role of DNA repair genes in individuals that are occupationally exposed to low dose radiation.