Mechanical stress activates platelets at a subhemolysis level: An in vitro study

BAKIR İ., Hoylaerts M. F., Kink T., Foubert L., Luyten P., Van Kerckhoven S., ...Daha Fazla

ARTIFICIAL ORGANS, cilt.31, sa.4, ss.316-323, 2007 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 4
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1111/j.1525-1594.2007.00381.x
  • Dergi Adı: ARTIFICIAL ORGANS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.316-323
  • Anahtar Kelimeler: free hemoglobin, hemoresistometer, platelet count, shear stress, sheep blood, RED-BLOOD-CELLS, SHEAR-STRESS, NATIVE BLOOD, ROLLER-PUMP, DAMAGE, FLOW, SUBENDOTHELIUM, AGGREGATION, CENTRIFUGAL, PARAFORMALDEHYDE
  • İstanbul Üniversitesi Adresli: Hayır

Özet

A feasibility study is performed to quantify sheep platelets (PLTs) and to identify the relationship between PLT count and hemolysis as a consequence of mechanical stress. Six adult, healthy Dorset sheep have been used for in vitro blood sampling test procedures in a hemoresistometer device (HRM). In each experiment, blood of the same animal was exposed to six different shear rates. Free hemoglobin levels and PLT count for each shear rate were detected. In all animals (A-F), hemolysis increased significantly between the shear rates of 2325 and 3100/s (P < 0.05) and the mean PLT count dropped immediately (contact, low shear) 40% in the beginning, between the shear rates of 0 and 775/s (P < 0.05). PLT count increased slightly as soon as hemolysis started. At higher shear rates, hemolysis increased and PLTs reduced further. Precise counting of PLTs indicates that PLTs are consumed dramatically at very low shear (by contact) and further by applied mechanical stress when hemolysis is obvious. A repetition of these tests with human blood could indicate species differences.