Analysis of the impact of indole-3-acetic acid (IAA) on gene expression during leaf senescence in Arabidopsis thaliana


Goren-Saglam N., Harrison E., Breeze E., Oz G., Buchanan-Wollaston V.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, vol.26, no.4, pp.733-745, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1007/s12298-019-00752-7
  • Journal Name: PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database
  • Page Numbers: pp.733-745
  • Keywords: Arabidopsis thaliana, IAA, Gene expression, Microarray, PSII activity, Leaf senescence, PROGRAMMED CELL-DEATH, CHLOROPHYLL FLUORESCENCE, TRANSCRIPTION FACTORS, PLANT SENESCENCE, OXIDATIVE STRESS, JASMONIC ACID, AUXIN, TOLERANCE, GROWTH, ETHYLENE
  • Istanbul University Affiliated: Yes

Abstract

Leaf senescence is an important developmental process for the plant life cycle. It is controlled by endogenous and environmental factors and can be positively or negatively affected by plant growth regulators. It is characterised by major and significant changes in the patterns of gene expression. Auxin, especially indole-3-acetic acid (IAA), is a plant growth hormone that affects plant growth and development. The effect of IAA on leaf senescence is still unclear. In this study, we performed microarray analysis to investigate the role of IAA on gene expression during senescence in Arabidopsis thaliana. We sprayed IAA on plants at 3 different time points (27, 31 or 35 days after sowing). Following spraying, PSII activity of the eighth leaf was evaluated daily by measurement of chlorophyll fluorescence parameters. Our results show that PSII activity decreased following IAA application and the IAA treatment triggered different gene expression responses in leaves of different ages.