A peroxiredoxin antioxidant is encoded by a dormancy-related gene, Per1, expressed during late development in the aleurone and embryo of barley grains

STACY R., MUNTHE E., Steinum T. M., SHARMA B., AALEN R.

PLANT MOLECULAR BIOLOGY, cilt.31, sa.6, ss.1205-1216, 1996 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 6
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1007/bf00040837
  • Dergi Adı: PLANT MOLECULAR BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1205-1216
  • Anahtar Kelimeler: late embryogenesis-abundant (lea), thiol-specific antioxidant (TSA), Hordeum vulgare L, desiccation tolerance, THIOL-SPECIFIC ANTIOXIDANT, ALKYL HYDROPEROXIDE REDUCTASE, VULGARE L SEEDS, AVENA-FATUA, HYDROGEN-PEROXIDE, GIBBERELLIC-ACID, SALMONELLA-TYPHIMURIUM, MOLECULAR-CLONING, OXIDATIVE DAMAGE, MULTIGENE FAMILY
  • İstanbul Üniversitesi Adresli: Hayır

Özet

Antioxidants can remove damaging reactive oxygen species produced as by-products of desiccation and respiration during late embryogenesis, imbibition of dormant seeds and germination. We have expressed a protein, PER1, encoded by the Balem (barley aleurone and embryo) transcript previously called B15C, and show it to reduce oxidative damage in vitro. PER1 shares high similarity to a novel group of thiol-requiring antioxidants, named peroxiredoxins, and represents a subgroup with only one conserved cysteine residue (l-Cys). PER1 is the first antioxidant belonging to the l-Cys subgroup shown to be functionally active, and the first peroxiredoxin of any kind to be functionally described in plants. The steady state level of the transcript, Per1, homologous to a dormancy-related transcript (pBS128) from bromegrass (Bromus secalinus), increases considerably in imbibed embryos from dormant barley (Hordeum vulgare L.) grains. Our investigations also indicate that Per1 transcript levels are dormancy-related in the aleurone layer of whole grains. In contrast to most seed-expressed antioxidants Per1 disappears in germinating embryos, and in the mature aleurone the transcript is down-regulated by the germinating embryo or by gibberellic acid (GA). Our data show that the barley seed peroxiredoxin is encoded by a single Per1 gene. Possible roles of the PER1 peroxiredoxin in barley grains during desiccation, dormancy and imbibition are discussed.