Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data

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Bengtsson-Palme J., Ryberg M., Hartmann M., Branco S., Wang Z., Godhe A., ...Daha Fazla

METHODS IN ECOLOGY AND EVOLUTION, cilt.4, sa.10, ss.914-919, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 10
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1111/2041-210x.12073
  • Dergi Adı: METHODS IN ECOLOGY AND EVOLUTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.914-919
  • İstanbul Üniversitesi Adresli: Hayır

Özet

The nuclear ribosomal internal transcribed spacer (ITS) region is the primary choice for molecular identification of fungi. Its two highly variable spacers (ITS1 and ITS2) are usually species specific, whereas the intercalary 5.8S gene is highly conserved. For sequence clustering and blast searches, it is often advantageous to rely on either one of the variable spacers but not the conserved 5.8S gene. To identify and extract ITS1 and ITS2 from large taxonomic and environmental data sets is, however, often difficult, and many ITS sequences are incorrectly delimited in the public sequence databases.