Akademik Veri Yönetim Sistemi
INTERNATIONAL JOURNAL OF SECONDARY METABOLITE, cilt.7, sa.2, ss.126-138, 2020 (Hakemli Dergi)
This study on Acer campestre L. subsp. campestre, used traditionally
in treatments of various inflammation disorders, especially hemorrhoids and
rheumatism, aimed to determine the total phenolic contents, the antioxidant and
antimicrobial potentials, and the chemical composition profile of the extracts and
subextracts from maple syrup and Acer campestre L. subsp. campestre leaves and
twigs. The antioxidant activities of maple syrup extracts and subextracts were
examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and the total phenolic
contents of all samples were determined using the Folin-Ciocalteu reagent (FCR)
method. Also, they were tested for their antimicrobial activities by microbroth
dilution technique. Catechic tannin, saponins and flavonoids are determined in its
leaves and twigs by preliminary qualitative phytochemical analysis. The
alcoholic extracts obtained by 48-hour maceration (91.2%), ethyl acetate
subextract of alcohol extracts obtained by 24-hour maceration (91.1%) and
alcohol extracts obtained by 24-hour maceration (88.8%) exhibited higher radical
scavenging activity than other samples, while the decoction and infusion had
moderate activities. Beside of this, it was found that the syrup and its subextracts
have more total phenolic contents than other extracts. The alcohol extracts
obtained by 24-hour maceration and by 48-hour maceration exhibited higher
activity against Candida albicans (78 μg/mL and 156 μg/mL, respectively). Only
the alcohol extract obtained by 48-hour maceration and ethyl acetate subextract
of syrup showed an activity against Escherichia coli, while all samples except
butanol subextract of syrup have an antibacterial activity against Pseudomonas
aeruginosa.