EFFECTS OF DIFFERENT DOSES OF TAURINE IN THE GLUCOSE-BASED EXTENDER DURING CRYOPRESERVATION OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) SEMEN


Ekici A., Baran A., Yamaner G., Ozdas O. B., Sandal A. I., Guven E., ...Daha Fazla

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.26, sa.4, ss.3113-3115, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.5504/bbeq.2012.0041
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3113-3115
  • Anahtar Kelimeler: Rainbow trout, Onchorhynchus mykiss, taurine, sperm, cryopreservation, LIPID-PEROXIDATION, SUPEROXIDE-DISMUTASE, OXIDATIVE STRESS, SPERM, ANTIOXIDANTS, HYPOTAURINE, SPERMATOZOA, FERTILITY, MOTILITY, PARAMETERS
  • İstanbul Üniversitesi Adresli: Evet

Özet

This study aimed to investigate the effects of different doses of taurine added to glucose extender Semen was collected from rainbow trout (Oncorhynchus mykiss) by abdominal massage and diluted (1:2) with 300 mM glucose (G) extender containing different taurine doses (50, 75 and 100 mM). The control group extender did not contain taurine. After dilution and dosing, samples were filled to 0.5 ml straws, frozen in nitrogen vapour and stored in liquid nitrogen. The comparison of the results obtained for the 50 mM taurine group and the control group showed no significant difference between the post-thawing motility percentages and motility duration of sperm, and only a very low statistically difference for eyed-embryo percentages (P < 0.05).