H3K9me3/H4K2Ome3 Ratio in Circulating Nucleosomes as Potential Biomarker for Colorectal Cancer

Deligezer U., Akisik E. Z. , Akisik E. E. , Kovancilar M., Bugra D., Erten N., ...More

6th International Conference on Circulating Nucleic Acids in Plasma and Serum, Hong Kong, PEOPLES R CHINA, 9 - 11 November 2009, pp.97-103 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume:
  • City: Hong Kong
  • Country: PEOPLES R CHINA
  • Page Numbers: pp.97-103
  • Keywords: Colorectal cancer, Circulating nucleosomes, Histone methylation, Plasma, Sat2 repeat, HISTONE MODIFICATIONS, METHYLATION STATES, HETEROCHROMATIN, CHROMATIN, REPEATS, DNA
  • Istanbul University Affiliated: Yes


Detection of cancer-related histone modifications of nucleosomes in the circulating blood may be useful in early cancer diagnosis. We have analyzed the trimethylation of histone H3 lysine 9 (H3K9me3) and histone H4 lysine 9 (H4K2Ome3), two modifications involved in heterochromatin formation, at pericentric heterochromatin of circulating nucleosomes in the blood plasma of healthy individuals, patients with either colorectal cancer or multiple myeloma. H3K9me3 was found to be decreased in colorectal cancer and increased in multiple myeloma while H4K2Ome3 levels were similar in all study groups. Therefore, we used the H3K9me3/H4K2Ome3 ratio for normalizing H3K9me3 concentrations; it significantly distinguished patients with colorectal cancer (median 0.8) from the healthy group (median 3) and multiple myeloma (median 4.7) (both p < 0.001). We conclude that if validated in a larger series of cases the ratio H3K9me3/H4K2Ome3 might be a potential diagnostic biomarker for colorectal cancer.