H3K9me3/H4K2Ome3 Ratio in Circulating Nucleosomes as Potential Biomarker for Colorectal Cancer


Deligezer U., Akisik E. Z., Akisik E. E., Kovancilar M., Bugra D., Erten N., ...Daha Fazla

6th International Conference on Circulating Nucleic Acids in Plasma and Serum, Hong Kong, PEOPLES R CHINA, 9 - 11 Kasım 2009, ss.97-103 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası:
  • Basıldığı Şehir: Hong Kong
  • Basıldığı Ülke: PEOPLES R CHINA
  • Sayfa Sayıları: ss.97-103
  • Anahtar Kelimeler: Colorectal cancer, Circulating nucleosomes, Histone methylation, Plasma, Sat2 repeat, HISTONE MODIFICATIONS, METHYLATION STATES, HETEROCHROMATIN, CHROMATIN, REPEATS, DNA
  • İstanbul Üniversitesi Adresli: Evet

Özet

Detection of cancer-related histone modifications of nucleosomes in the circulating blood may be useful in early cancer diagnosis. We have analyzed the trimethylation of histone H3 lysine 9 (H3K9me3) and histone H4 lysine 9 (H4K2Ome3), two modifications involved in heterochromatin formation, at pericentric heterochromatin of circulating nucleosomes in the blood plasma of healthy individuals, patients with either colorectal cancer or multiple myeloma. H3K9me3 was found to be decreased in colorectal cancer and increased in multiple myeloma while H4K2Ome3 levels were similar in all study groups. Therefore, we used the H3K9me3/H4K2Ome3 ratio for normalizing H3K9me3 concentrations; it significantly distinguished patients with colorectal cancer (median 0.8) from the healthy group (median 3) and multiple myeloma (median 4.7) (both p < 0.001). We conclude that if validated in a larger series of cases the ratio H3K9me3/H4K2Ome3 might be a potential diagnostic biomarker for colorectal cancer.