Akademik Veri Yönetim Sistemi
III. International Agricultural, Biological & Life Science Conference, Edirne, Türkiye, 29 - 31 Ağustos 2022, ss.268
Stress conditions which affect protein folding cause Endoplasmic Reticulum (ER) stress. This
will be transferred to the nucleus by Unfolded Protein Response (UPR), which is a cellular
homeostatic response against ER stress. Diversity of ER-stress associated genes, which were
elucidated by genome sequencing studies of various plants, proved that plants use ER stress
responses to better adapt to their environment more than animals. Tunicamycin, as an N-linked
glycosylation inhibitor, is a widely known and used chemical to induce ER stress. In this study,
tunicamycin was used to induce ER stress in tomato plants. Total RNA was isolated from plant
leaves to perform miRNA expression analysis in stress-induced plants. The miRNAs selected
for this study were studied in various plants under various stress conditions, and they were
proven to show changing expression rates. It is important to reveal the relationship between ER
stress and expression profiles of these miRNAs, which were studied under different stress
conditions, by evaluating the data obtained in this study. Expression changes of these miRNAs,
which are known to be associated with stress responses and are observed to change under many
stress conditions in tomato, were investigated by qRT-PCR method under ER stress. It is
anticipated that the obtained data will contribute to the elucidation of key miRNAs involved in
ER stress response mechanisms.