Evaluation of the androgenic competence of 66 wild Turkish Vaccaria hispanica (Mill.) Rauschert genotypes through microspore culture


ARI E., Bedir H., DENİZ İ. G., GENÇ İ., Seguí-Simarro J. M.

Plant Cell, Tissue and Organ Culture, vol.148, no.1, pp.209-214, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 148 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.1007/s11240-021-02169-1
  • Journal Name: Plant Cell, Tissue and Organ Culture
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Compendex, Environment Index, Food Science & Technology Abstracts, Veterinary Science Database
  • Page Numbers: pp.209-214
  • Keywords: Callus, Cow cockle, Endophyte, Hairy root, Haploid, Microspore culture, GROWTH
  • Istanbul University Affiliated: Yes

Abstract

© 2021, The Author(s), under exclusive licence to Springer Nature B.V.One of the most remarkable natural plants is Vaccaria hispanica (Mill.) Rauschert, which has a high economic, medicinal and industrial potential due to its valuable compounds. However, it is mostly an underused plant worldwide. To implement doubled haploid technology in plant breeding programs and exploit its potential, first knowing the particulars of the species is necessary. This study was aimed to explore the androgenic potential of different wild Turkish V. hispanica genotypes collected from different Turkish regions, as a starting point to identify bottlenecks to solve in future studies. As to induction of microspore embryogenesis, nearly all of them responded, with efficiencies reaching 300 embryos/100 buds in some cases. However, we also found three main bottlenecks. First, the presence of foam-producing saponins in flowers prevented an efficient isolation of microspores. Second, embryos showed a reduced ability to germinate. Third, a dense network of hairy roots prevented the formation of a true, fully functional root system. Together, these drawbacks led to the production of very few DH plants. The presence of rhizogenic endophytes may be the cause of most of these problems, which opens the door for possible solutions.