Global quantification of heterochromatin-associated histone methylations in cell lines with differential sensitivity to ionizing radiation

Cetinkaya M., Ozgur E., Dalay N., Gezer U.

ACTA BIOCHIMICA POLONICA, cilt.62, sa.2, ss.173-176, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.18388/abp.2014_790
  • Dergi Adı: ACTA BIOCHIMICA POLONICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.173-176
  • İstanbul Üniversitesi Adresli: Evet

Özet

Histone modifications are involved in the DNA damage response (DDR). Here, by utilizing an ELISA immunoassay we assessed the methylation at H3K9 (H3K9me2 and H3K9me3) in two cell lines with differential sensitivity to radiation-induced apoptosis, He La (sensitive) and MCF-7 (resistant). We found that DNA damage induction by gamma-irradiation leads to considerable accumulation (up to 5-fold) of H3K9me2 and H3K9me3, but not of H4K20me3 (control modification) in MCF-7 cells (p<0.05). Interestingly, a lower dose (2 Gy) was more effective than 5 Gy. In He La cells a smaller effect (approx. 1.5-1.8-fold) was evident only at 5 Gy. In conclusion, our findings reveal that DNA damage leads to specific accumulation of H3K9me2 and H3K9me3 in a cell-type specific manner.