Novel electrochemical biosensor forEscherichia coliusing gold-coated tungsten wires and antibodyfunctionalized short multiwalled carbon nanotubes

Ertaş T., Dinç B., Üstünsoy R., Eraslan H., Ergenç A. F., Bektaş M.

INSTRUMENTATION SCIENCE AND TECHNOLOGY, vol.2023, pp.1-16, 2023 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 2023
  • Publication Date: 2023
  • Doi Number: 10.1080/10739149.2023.2222801
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, Compendex, INSPEC
  • Page Numbers: pp.1-16
  • Istanbul University Affiliated: Yes


Rapid and easy detection of pathogens, especially bacteria, playsan important role in daily life in the face of increasing environ-mental pollution. Nanosensors focus on carbon nanotubes, whichcan bind various molecules to large surfaces and have high elec-tron conductivity. They are widely used in the development ofelectrochemical biosensors. In this study, a new design of electro-chemical biosensors is presented by integrating gold-coated tung-sten wires (GC-TW) and acid-functionalized multi-walled carbonnanotubes (MWCNTs) in a two-step coating process. GC-TWs arecoated with acid-functionalized short MWCNTs. Fourier transformsinfrared spectroscopy (FTIR), transmission electron microscopy(TEM), and scanning electron microscopy (SEM) results show effi-cient immobilization of the electrodes. The performance forEscherichia  coli(E.  coli) K-12 is evaluated based upon the changein current following antibody-antigen interactions. Bioimpedancemeasurements are performed from 50 Hz to 10 MHz to achieveoptimal coverage of impedance changes. The measurementsshow that the electrode has a detection limit of 0.8 CFU/mL. Theresponse of the electrode saturates when the bacterial concentra-tion is increased to 1.70 x 102CFU/mL.ThebiosensoriscapableofdetectingE.  colibetween 0.8 and 1.70102CFU/mL. The datashow that this MWCNT-based biosensor can be developed into ahighly sensitive device forE. coli.