Molecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for ELISA applications

Alkanli S. S., Dal Yontem F., Yasar M., Guven C., KAHRAMAN M. V., KAYAMAN APOHAN N., ...More

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.34, no.6, pp.753-767, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 6
  • Publication Date: 2023
  • Doi Number: 10.1080/09205063.2022.2145866
  • Journal Name: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.753-767
  • Keywords: Molecularly imprinted polymer, plastic antibody, diphtheria toxin, ELISA, CATALYTIC DOMAIN, TRANSLOCATION, PROTEINS
  • Istanbul University Affiliated: Yes

Abstract

Plastic antibodies can be used for in vitro neutralization of biomacromolecules with different fragments due to their potential in separation, purification, chemical sensor, catalysis and drug production studies. These polymer nanoparticles with binding affinity and selectivity comparable to natural antibodies were prepared using functional monomer synthesis and copolymerization of acrylic monomers via miniemulsion polymerization. As a result, the in vitro cytotoxic effect from diphtheria toxin was reduced by MIPs. In vitro imaging experiments of polymer nanoparticles (plastic antibodies) were performed to examine the interaction of diphtheria toxin with actin filaments, and MIPs inhibited diphtheria toxin damage on actin filaments. The enzyme-linked immunosorbent assay (ELISA) was performed with plastic antibodies labeled with biotin, and it was determined that plastic antibodies could also be used for diagnostic purposes. We report that molecularly imprinted polymers (MIPs), which are biocompatible polymer nanoparticles, can capture and reduce the effect of diphtheria toxic and its fragment A.