Role of SNPs of <i>CPTIA</i> and <i>CROT</i> genes in the carnitine-shuttle in coronary artery disease: a case control study


Demircan A., Coskunpinar E., Kanca D., Ozkara G., Yanar F., Kurnaz-Gomleksiz O., ...Daha Fazla

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.44, sa.6, ss.822-830, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 6
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1515/tjb-2018-0546
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.822-830
  • Anahtar Kelimeler: CPTIA, CROT, Gene, Long-chain fatty acids, beta oxidation, Cholesterol, Coronary artery disease, FATTY-ACID OXIDATION, ISOFORM DEFICIENCY, HEART-DISEASE, PALMITOYLTRANSFERASE, POLYMORPHISMS, LETHAL, MUSCLE
  • İstanbul Üniversitesi Adresli: Evet

Özet

Objective: Fatty acid beta-oxidation defects can lead to difficulties at covering energy requirement of heart. The carnitine-shuttle is responsible for the transfering of long-chain fatty acids from the internal mitochondrial membrane. The role of genetic variants of the enzymes in the carnitine shuttle in coronary artery disease (CAD) has not been studied. Therefore, we performed a case-control study investigating the possible relation between the CPTIA-rs3019613 and CROT-rs2214930 gene variations located carnitine shuttle and CAD risk. Materials and methods: Study groups were comprised of 96 CAD patients and 85 controls. CPTIA-rs3019613 G >A and CROT-rs2214930 T>C polymorphisms were determined by real-time-PCR. Results: The CROT-rs2214930-CC genotype was found to be associated with decreased HDL-cholesterol (HDL-C) in controls (p = 0.029). In patients with CPTIA-rs3019613-A allele, body mass index (BMI) (p = 0.016) and BMI threshold-value (p = 0.030) were found be higher compared to those with GG-genotype, while HDL-C threshold-value (HDL-C <= 0.90 mmol/L) was found to be lower (p = 0.015). Regression analysis confirmed CPTIA-rs3019613-A allele has a significant relationship with decreased HDL-C (p= 0.009) in patients. Conclusion: Our study indicated that the polymorphisms of the CROT and CPTIA genes related to beta-oxidation of long-chain fatty acids had an important effect on serum HDL-C levels and may be a potential risk for CAD.