Biosynthesis of Silver Nanoparticles Using White Propolis Extract as a Reduction Agent and Optimized by Box-Behnken Design

Goksen Tosun N., Kaplan O.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, vol.25, no.5, pp.933-945, 2022 (ESCI) identifier identifier


In this study, it was aimed to optimize the biosynthesis of silver nanoparticles with a Box-Behnken design. The white propolis extract was utilized as the reduction and stabilizing agent. The synthesized silver nanoparticles using white propolis extract solution were optimized by Box-Behnken design considering the effect of certain independent variables such as microwave power, time, and concentration of AgNO3 (silver nitrate). A quadratic polynomial model was used in mathematical modeling and response surface analysis was performed to determine the independent variable -response relationship. The optimum synthesis conditions were determined as 10 mM of AgNO3 concentration, 0.3 of VExt/VAg, 150 watts of microwave power, and 35 seconds. The optimized silver nanoparticles were characterized using FTIR (Fourier Infrared) spectroscopy, UV-VIS (Ultraviolent visible) spectrophotometry, and DLS (Dynamic Light Scattering). In addition, the antibacterial activity of the optimized silver nanoparticles was tested against Staphylococcus aureus (S. aureus), Klebsiella pneumonia (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), and Enterococcus faecalis (E. faecalis) strains. It was observed that synthesized silver nanoparticles had higher antibacterial activity compared to propolis extract.