Development of piperine nanoemulsions: an alternative topical application for hypopigmentation.

Ozkan B., Altuntas E., Cakir Koc R., Budama-Kilinc Y.

Drug development and industrial pharmacy, cilt.48, sa.3, ss.117-127, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1080/03639045.2022.2100901
  • Dergi Adı: Drug development and industrial pharmacy
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CAB Abstracts, Chemical Abstracts Core, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.117-127
  • Anahtar Kelimeler: Piperine, vitiligo, hypopigmentation, topical, nanoemulsion, melanogenesis, tyrosinase activity, IN-WATER NANOEMULSIONS, ULTRASONIC EMULSIFICATION, PHYSICAL STABILITY, PROCESS PARAMETERS, FORMULATION, DELIVERY, EMULSIONS, VITILIGO, EXTRACT, QUALITY
  • İstanbul Üniversitesi Adresli: Evet

Özet

This study, it was aimed to develop a topical piperine nanoemulsion (P-NE) using an ultrasonic emulsification process to find an alternative treatment option for some hypopigmentation disorders such as vitiligo. Results showed that 150 mg piperine loaded NE with 1:2 oil phase to S-mix ratio and manufactured with 20 min ultrasonication duration with the pre-emulsification step was the most durable formulation with a mean globule size of 216.00 +/- 2.65, a PdI value of 0.094 +/- 0.02 and a zeta potential value of -27.50 +/- 2.48 mV. After three months of storage, the selected P-NE (coded as F3P2) remained kinetically stable without visual changes. This formulation displayed a sustained release pattern with a release of 81.92% +/- 3.04% piperine after 72 h. According to our in vitro activity experiments, it was determined that the P-NE had no toxic effect including the dose of 5 mg/mL, and the highest P-NE formulation dose of 5 mg/mL increased tyrosinase activity by 32.77% +/- 9.09% and melanogenesis activity by 34.90% +/- 0.73%. In conclusion, it was demonstrated that the P-NE formulation may serve as a promising therapy for the efficient treatment of vitiligo. Moreover, P-NE formulation may also help in preventing irregular pigmentation and skin cancer, associated with the conventional treatment methods.