Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS


Bektaş N. Y., Ersoy E., Boǧa M., Boran T., Çinar E., ÖZHAN G., ...More

Turkish Journal of Chemistry, vol.45, no.5, pp.1621-1638, 2021 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.3906/kim-2104-17
  • Journal Name: Turkish Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1621-1638
  • Keywords: Hypericum androsaemum, cytotoxic, apoptosis, quillaic acid, antioxidant, LC-HRMS, BERRY-LIKE FRUITS, ANTIOXIDANT ACTIVITY, IN-VITRO, CANCER CELLS, BIOLOGICAL-ACTIVITIES, CHEMICAL-COMPOSITION, POLYPHENOL CONTENTS, WATER EXTRACT, CONSTITUENTS, L.
  • Istanbul University Affiliated: Yes

Abstract

© 2021 TUBITAK. All rights reserved.The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatographyhigh resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 ± 70.10 μg/g) in the leaves dichloromethane (LD), herniarin (167 ± 1.50 μg/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 ± 235.36 μg/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 ± 20.46 μg/g) in the fruit methanol (FM), and caffeic acid (370 ± 8.88 μg/g) in the fruit water (FW), and (3313 ± 79.51 μg/g) in the leaves water (LW) extracts. LM exerted strong antioxidant activity in DPPH free (IC5010.94 ± 0.08 μg/mL), and ABTS cation radicals scavenging (IC509.09 ± 0.05 μg/mL) activities. FM exhibited cytotoxic activity with IC50values of 73.23 ± 3.06 μg/mL and 31.64 ± 2.75 μg/mL on PC-3 and Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 ± 18.9 μg/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction ability on different cells, FM extract showed apoptosis induction activity with 64.75% on PC-3 cells at 50 μg/mL concentration. The study provides promising results about the potential of Hypericum androsaemum on cancer prevention.