Importance Of Polynnorphisms Of The MIF Gene At Acute Leukemia And Its Effect On Febrile Neutropenic Attacks

Guven S., PEHLİVAN M., YILMAZ M. B., Okan V., PEHLİVAN S.

EUROPEAN JOURNAL OF THERAPEUTICS, cilt.15, sa.2, ss.5-9, 2009 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2009
  • Dergi Adı: EUROPEAN JOURNAL OF THERAPEUTICS
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.5-9
  • Anahtar Kelimeler: Macrophage Migration Inhibitory Factor Gene, MIF-173 G/C polymorphism, PCR, Acute Leukemia, DNA
  • İstanbul Üniversitesi Adresli: Evet

Özet

The objective of this study is to research polymorphisms in MIF gene -173 site in variable haemotological malignities and evaluate its effects on febrile neutropenic attacks after chemotherapy in acute leukemia, Single nucleotide polyrnorphisms (SNPs) a-173 site of the MIF gene was analysed with the PCR-RFLP method (restriction endonudease Alu I) with isolated DNA samples from 48 patients who were treated for ALL and AML and 53 healthy controls, and G and C allel frequencies were compared statistically between patients and healthy controls groups, As a result of the analyzis, it was observed that the rate of patients with GG genotypes was 70.9%, with GC genotypes 29.1%, and with CC genotypes 0%, Association is evaluated between MIF pozItivities in acute leukemias and distribution of infections, its factors, duration of feveral days and neutropenia. Within the GC allel group; no significant differences were observed (p>0.05) in infection factors, duration of neutropenia (<500/L), duration of feveral days, fungal pnomoni, blood infections and FN mortality compared to the GG allel group. In conclusion, fungal pneumonia was observed at a higher rate in the CG allel group (22%) cumpared to the GG allel group (11.5%); a repeat of the study with an expended group can yield more information about this relationship.