Akademik Veri Yönetim Sistemi
CED/NOF IADR Oral Health Research Congress, Rodos, Yunanistan, 21 - 23 Eylül 2023, ss.131
Objectives: Theaflavins have been found to have inhibitory activity against collagen-degradable enzymes thus, they may be effective while dentin demineralization occurs. Since varnishes are more retentive than gels and solutions, the purposes of the present in-vitro study are to measure the inhibitory effect of experimental theaflavin (2a/2b) containing varnishes on dentin losses and evaluate their effect against matrixmetalloproteinases.
Methods: Dentin bloc samples (3×5×2 mm) were provided from human non-erupted third molars and were demineralized with citric acid (CA-0.3%) for 60 seconds to simulate dentinal erosive lesions. Then samples were allocated to six experimental and a control groups according to the material combinations (n=6):
1)Varnish A+TF2a, 2)Varnish B+TF2a, 3)Varnish C+TF2a , 4)Varnish A+TF2b, 5)Varnish B+TF2b, 6)Varnish C+TF2b and 7)Fluoride varnish as control. The varnishes were applied over the surfaces using a micro brush. After immersion in artificial saliva for 16 hours, samples were subjected to pH cycling using CA for 5 minutes for 5 days. Then the lesion area, depth, and mineral ratio were measured with micro-computed tomography (Micro-CT), and the effect of varnishes against MMPs was evaluated with Western Blot (WB) analysis. Obtained data were evaluated with One-way ANOVA at a significance of p>0.05.
Results: MicroCT analyses showed that mean lesion depth and mineral ratios were significantly reduced in experimental groups compared to the control group (p< 0.05). Lesion areas of the experimental groups were not significantly different from each other but, the control group presented significantly lower lesion depth
(p<0.05). WB analysis showed significantly lower MMP-2 levels in experimental groups than in the control
(p<0.05). There were no statistically significant MMP-9 levels among the groups tested (p>0.05).
Conclusions: Dental varnishes containing theaflavin 2a and 2b showed promising results in reducing dentinal wear and collagen degradation, likely due to their anti-MMP action. Therefore, using theaflavin varnishes may be suggested as natural-based agents for preventing dentin erosion.