miR-16-5p/CCNE1 Relation in AML Cells


Teomete Ş., Kaya M., Suer İ., Çefle K., Palanduz Ş., Öztürk Ş.

VIII. INSAC International Congress on Health Sciences (ICHES-2022), Konya, Turkey, 18 - 20 March 2022, pp.116-122

  • Publication Type: Conference Paper / Full Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.116-122
  • Istanbul University Affiliated: Yes

Abstract

Acute myeloid leukemia (AML) is a malignant and clonal hematological disease characterized by the abnormal accumulation of blast cells in the bone marrow and peripheral blood. miRNAs have been found to play a crucial role in almost all aspects of AML development, including proliferation, differentiation, and survival. This research aimed to elucidate the effects of miR-16-5p on AML cell lines via targeting oncogenic cell-cycle genes CCNE1 and CCND2. For this purpose, miRNA mimic transfection, qRT-PCR, and WST-8 assay methods were performed. Consequently, compared to the control group, the decline in the proliferation of miR-16-5p transfected cells was observed in cells. In comparison with the control group, the relative expression of the CCNE1 gene was found to be statistically significantly decreased in HL-60 cells transfected with miR-16-5p. Therefore, the present study suggests that the upregulation of miR-16-5p may inhibit the proliferation of HL-60 cells via targeting the CCNE1 gene.