Zinc oxide nanoparticles induced cyto- and genotoxicity in kidney epithelial cells


Uzar N., Abudayyak M. F., Akcay N., Algun G., Ozhan G.

TOXICOLOGY MECHANISMS AND METHODS, vol.25, no.4, pp.334-339, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 4
  • Publication Date: 2015
  • Doi Number: 10.3109/15376516.2015.1045654
  • Journal Name: TOXICOLOGY MECHANISMS AND METHODS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.334-339
  • Keywords: Cytotoxicity, genotoxicity, nanoparticle, nephrotoxicity, zinc oxide, ZNO NANOPARTICLES, OXIDATIVE STRESS, DNA-DAMAGE, TISSUE DISTRIBUTION, TITANIUM-DIOXIDE, CANCER-CELLS, COMET ASSAY, CYTOTOXICITY, APOPTOSIS, MUTAGENICITY
  • Istanbul University Affiliated: Yes

Abstract

The wide uses of zinc oxide nanoparticles (nano-ZnO) in industrial, cosmetics, medicine, food production and electronics associate with increase in occupational and public exposure. Although, toxicity of nano-ZnO has been extensively studied on many different cell types and animal systems, there is a significant lack of toxicological data focus on nephrotoxic potential of nano-ZnO. In this study, the cyto- and genotoxic effects of nano-ZnO on rat kidney epithelial cells (NRK-52E) were investigated by using different assays. Nano-ZnO (10-50nm of sizes) were synthesized by sol-gel method. For the cytotoxic effect of nano-ZnO, mean of inhibition concentration (IC50) values in cell line was evaluated by MTT, Trypan Blue (TB) and Neutral Red Uptake (NRU) assays at 25.0-100.0 mu g/mL exposure concentrations. Nano-ZnO showed cytotoxic activity by acting on different targets in renal cells, with IC50 >= 73.05 mu g/mL. Comet assay was used to evaluate the genotoxicity of nano-ZnO (12.5-50.0 mu g/mL). Nano-ZnO caused statistically significant DNA damage. Our results highlight the important risk of cyto-and genotoxic effects of nano-ZnO over the kidney.