In vitro effects of peroxynitrite on human spermatozoa

OZTEZCAN S., Turkoglu U., KERVANCIOGLU E., Kocak T., Kocak-Toker N., AYKAC-TOKER G.

ANDROLOGIA, cilt.31, sa.4, ss.195-198, 1999 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 4
  • Basım Tarihi: 1999
  • Doi Numarası: 10.1046/j.1439-0272.1999.00279.x
  • Dergi Adı: ANDROLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.195-198
  • Anahtar Kelimeler: peroxynitrite, infertility, lipid peroxidation, sulfhydryl content, sperm motility, NITRIC-OXIDE SYNTHASE, LIPID-PEROXIDATION, SUPEROXIDE-DISMUTASE, SPERM MOTILITY
  • İstanbul Üniversitesi Adresli: Evet

Özet

In the present study, the in vitro effects of peroxynitrite on sperm motility, lipid peroxidation and sulfhydryl content were examined. Sperm percentage motility and movement characteristics were assessed by a computer-assisted system. Lipid peroxidation was measured by determining malondialdehyde levels using the thiobarbituric acid (TBA) method. Sperm sulfhydryl content was measured by a spectrophotometric method based on reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) by sulfhydryl groups. Percentage motility, movement characteristics and sulfhydryl content decreased significantly in peroxynitrite-treated samples compared to decomposed peroxynitrite-treated samples. Lipid peroxidation in peroxynitrite-treated samples was significantly higher than in decomposed peroxynitrite-treated samples. These results indicate that peroxynitrite anion may cause sperm dysfunction through lipid peroxidation stimulation and total SH depletion.