In vitro activities of antibiotic combinations against mature biofilms of ventilator-associated pneumonia isolates

Copur B., Dosler S., Aktas Z., Basaran S., Simsek-Yavuz S., Cagatay A., ...More

FUTURE MICROBIOLOGY, vol.17, no.13, pp.1027-1042, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 13
  • Publication Date: 2022
  • Doi Number: 10.2217/fmb-2021-0305
  • Journal Name: FUTURE MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1027-1042
  • Keywords: biofilm, checkerboard, synergy, time-kill curve, ventilator-associated pneumonia, ENDOTRACHEAL-TUBE BIOFILM, PSEUDOMONAS-AERUGINOSA, MEROPENEM, COLISTIN, CIPROFLOXACIN, MICROBIOLOGY, INFECTION, PATHOGENS, SYNERGY, DEVICE
  • Istanbul University Affiliated: Yes

Abstract

Background: The authors aimed to determine the efficacy of frequently used antibiotics, alone or in combination, against biofilms of ventilator-associated pneumonia isolates. Materials & methods: The authors determined the MICs, minimum biofilm inhibitory concentrations and minimum biofilm eradication concentrations of meropenem, ciprofloxacin and colistin as well as their combinations against planktonic forms and biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii clinical isolates. Results: Generally, the minimum biofilm inhibitory concentrations and minimum biofilm eradication concentrations of the antibiotics were 1000-fold higher than their MICs, and synergy was provided by different concentrations of meropenem-colistin and meropenem-ciprofloxacin combinations with checkerboard and time-kill curve methods. Conclusion: The combination of meropenem and ciprofloxacin seems to be a good candidate for the treatment of biofilm-associated infections; none of the concentrations obtained as a result of the synergy test were clinically significant.