Assessment of circulating serum DNA integrity in colorectal cancer patients.


Yoruker E. E. , Ozgur E. , Keskin M. , Dalay N., Holdenrieder S., Gezer U.

Anticancer research, vol.35, no.4, pp.2435-40, 2015 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 4
  • Publication Date: 2015
  • Title of Journal : Anticancer research
  • Page Numbers: pp.2435-40
  • Keywords: colorectal cancer, circulating cell-free DNA, DNA integrity, circulating nucleosomes, CELL-FREE DNA, PLASMA, NUCLEOSOMES, BIOMARKER, ORIGIN, BLOOD

Abstract

Background/Aim: Studies evaluating the integrity of cell-fee DNA (cfDNA) in colorectal cancer (CRC) have led to inconsistent results. Herein, we analyzed the utility of two different DNA integrity indexes (ACTB(384)/ACTB(106) and ALU(247)/ALU(115)) to assess cfDNA fragmentation in Turkish CRC patients. The correlation of circulating nucleosomes (cNUC) with the fragment sizes was also evaluated. Materials and Methods: Seventy two CRC patients and 42 CRC-free control individuals were enrolled in the study. cfDNA was analyzed by quantitative polymerase chain reaction (qPCR). Results: While with ALU(247)/ALU(115) there was a small difference between the groups, an approximately 3-fold (median values=0.12 and 0.34) lower integrity was found in the patients using the ACTB(384)/ACTB(106) ratio (p=0.06). The correlation between cNUC and qPCR threshold cycles was much higher for shorter fragments. Conclusion: Lower DNA integrity and the predominance of mononuclesomes in serum reveal high fragmentation of cfDNA in CRC patients.