Identification of a differential expression signature associated with tumorigenesis and metastasis of laryngeal carcinoma

Coskunpinar E., Oltulu Y. M., Orhan K. S., Tiryakioglu N. O., Kanliada D., AKBAŞ F.

GENE, cilt.534, sa.2, ss.183-188, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 534 Sayı: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.gene.2013.10.063
  • Dergi Adı: GENE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.183-188
  • Anahtar Kelimeler: TIMP1, c-Myc, SPARC, COL4A2, TGFB1, SOX4, SQUAMOUS-CELL CARCINOMAS, C-MYC, MICROARRAY ANALYSIS, GENE-EXPRESSION, CANCER CELLS, HUMAN-PAPILLOMAVIRUS, TISSUE INHIBITORS, MATRIX PROTEIN, IV COLLAGEN, HUMAN SOX4
  • İstanbul Üniversitesi Adresli: Evet

Özet

Objectives: Metastasis is the most significant prognostic factor for laryngeal carcinoma which necessitates the identification of molecular alterations associated with metastasis. The identification of such molecular alterations will not only prove useful in treatment but also provide insight into mechanisms of cancer metastasis. The studies conducted so far have not specifically focused on metastasis or invasion pathways. Therefore we investigated the expression profiles with a pathway focused approach.

Abstract

OBJECTIVES:

Metastasis is the most significant prognostic factor for laryngeal carcinoma which necessitates the identification of molecular alterations associated with metastasis. The identification of such molecular alterations will not only prove useful in treatment but also provide insight into mechanisms of cancer metastasis. The studies conducted so far have not specifically focused on metastasis or invasion pathways. Therefore we investigated the expression profiles with a pathway focused approach.

MATERIALS AND METHODS:

Total RNA was extracted from 36 laryngeal tumors and paired cancer free tissue. Expression levels of 88 genes were determined using a PCR array system following cDNA synthesis. Obtained data was used for the calculation of altered expression levels, facilitating relevant algorithms. Significant alterations were determined according to their p-value obtained by Student's t-test.

RESULTS:

Sixteen genes have shown altered expression when compared with adjacent cancer-free tissue. 2 of these 16 genes have shown differential expression in tumors with neck metastasis in respect to non-metastatic tumors.

CONCLUSION:

We found that TGFB1, TIMP1, c-Myc, SPARC, COL4A2 and SOX4 show altered expression in laryngeal tumors. c-Myc and SOX4 expression is decreased as laryngeal tumors switch to metastatic phenotype.

Copyright © 2013 Elsevier B.V. All rights reserved.

KEYWORDS:

COL4A2; FDR; GSEA; HPV; Human papillomavirus; LSCC; PCR; RNA; SOX4; SPARC; SRY-related HMG-box-4; TGFB1; TIMP1; Tissue Inhibitor of Metalloproteinase I; Transforming Growth Factor Beta I; c-Myc; cDNA; collagen alpha-2(IV); complementary deoxyribonucleic acid; false discovery rate; gene set enrichment analysis; laryngeal squamous cell carcinoma; polymerase chain reaction; qPCR; quantitative polymerase chain reaction; ribonucleic acid; secreted protein acidic and rich in cysteine; v-myc avian myelocytomatosis viral oncogene homolog