Purpose: This study aimed to measure the performance of cervical multimodal hyperspectroscopy (MHS) when compared to Pap smear, and HPV testing in low-risk pregnant women followed up in a university clinical setting. Materials and Methods: Each of 91 pregnant women underwent Pap smear, HPV testing, and during the fast 20 weeks of pregnancy. In 84 subjects evaluable data were collected and analyzed. Results: Average age of women was 30 +/- 5.2 years. The specificity of MHS performed in pregnant women seems to be significantly better (p < 0.01) than the data of non-pregnant women published earlier. The specificity of MHS in pregnant women with normal Pap smear result is estimated to be 92.9%. The procedure was also well-tolerated with a patient satisfaction rate of 92%. Conclusions: Scanning of the cervix by MIIS may perform similar to Pap smear + ITV testing and may have a higher specificity in low-risk pregnant women.