Akademik Veri Yönetim Sistemi
European Human Genetics Conference Hybrid Conference Milan, Italy May 24–27, 2025, Milan, İtalya, 24 - 27 Mayıs 2025, ss.3033, (Özet Bildiri)
Background: MicroRNAs (miRNAs) are small non-coding RNAs that play a critical role in post-transcriptional gene regulation and have emerged as promising biomarkers for several diseases. In pregnancies with pathological ultrasound findings of the fetus, amniotic fluid (AF) miRNAs might be informative for the underlying genetic factors. This study aims to analyze and compare the AF cell-free miRNAs and AF exosomal miRNAs in renal abnormalities of the fetus.Materials and Methods: Published data (n=40) was systematically analyzed and AF cell-free miRNAs and exosomal miRNAs associated with fetal renal abnormalities were curated. mRNA targets were predicted and only the common targets detected by three prediction tools (Targetscan v7.1, Pictar, miRBase v22.1) were further analyzed. Subsequently, the mRNAtargets associated with renal function and abnormalities were selected. The cellular function andassociated pathways of the target mRNAs are evaluated and compared between cell-free and exosomal miRNAs.Results: In published data, among AF cell-free miRNAs, hsa-let-7f, hsa-miR-155, hsa-miR-210, hsa-miR-200, hsa-miR-19, hsa-miR-92a, miR-30a and among the AF exosomal miRNAs hsa-let-7f, hsa-miR-299-5p, hsa-miR-195-5p, hsa-miR-300 and, hsa-miR-381 were associated with renal abnormalities. The predicted mRNA targets were found to be involved in pathways such as protein binding, DNA regulation, transcription, ATP binding, and methionine adenosyltransferase activity.Conclusion: This study shows that although there were common miRNAs between the AF cell-free and exosomal miRNAs in renal abnormalities, the detected miRNAs diverged between thetwo groups. However, the study is limited by the detection methods of the analyzed publications and detected miRNAs should be evaluated by further functional studies.