Endothelium-dependent vasorelaxant effect of Alchemilla vulgaris methanol extract: a comparison with the aqueous extract in rat aorta

Takir S., Sezgi B., Suzgec-Selcuk S., Eroglu-Ozkan E., Beukelman K. J., Mat A., ...Daha Fazla

NATURAL PRODUCT RESEARCH, cilt.28, sa.23, ss.2182-2185, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 23
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1080/14786419.2014.926352
  • Dergi Adı: NATURAL PRODUCT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2182-2185
  • Anahtar Kelimeler: Alchemilla vulgaris extract, rat aorta, relaxation, endothelium, flavonoid, quercetin, SMOOTH-MUSCLE, GALLIC ACID, FLAVONOIDS
  • İstanbul Üniversitesi Adresli: Evet

Özet

We aimed to investigate the vascular effects of methanol extract (ME) and aqueous extract (AE) of Alchemilla vulgaris (Rosaceaea). Increasing concentrations of the ME (0.01-10 mg/mL) produced relaxations in noradrenaline (NA: 10(-6) M) and K+ (40mM) precontracted aortas while contractions were obtained with the AE (0.01-10 mg/mL). Responses to the ME were inhibited in the presence of putative inhibitors of endothelial vasodilators or after removal of the endothelium. Pretreatment of aortic rings with the ME (10 mg/mL, 20 min) reduced the maximal contractions to NA and K+, whereas an enhanced contractility was observed with the AE (10 mg/mL, 20 min). Total flavonoid content was higher in the ME than in the AE. Quercetin was determined particularly high in the ME while gallic acid was high in the AE. Our results indicated that the ME of A. vulgaris displays favourable vascular effects via endothelium-dependent mechanisms.