POTENTIATION OF ETOPOSIDE-INDUCED DNA-DAMAGE BY VERAPAMIL IN L-STRAIN CELLS IN-VITRO


RIDVANOGULLARI M., AYDINER A., ANIL D., SAYIN U., DINCOL K., TOPUZ E., ...More

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, vol.12, no.4, pp.255-260, 1993 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 4
  • Publication Date: 1993
  • Journal Name: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, MEDLINE, Directory of Open Access Journals
  • Page Numbers: pp.255-260
  • Istanbul University Affiliated: Yes

Abstract

Verapamil has previously been shown to have enhanced the cytotoxicity of VP-16-213 against malign cell lines in vitro. In this study the cytotoxic effects of verapamil on the cytotoxicity of etoposide against transformed mouse fibroblasts (L-strain cell) were examined. When verapamil was used alone, after 8 hrs, DNA synthesis was increased with respect to the control cells (p<0.01) and the initial increase of DNA synthesis was higher with the lowest concentration of verapamil (2 mu g/ml) (p<0.01). In cells that were treated with verapamil and etoposide, after 16 hrs, etoposide cytotoxicity was enhanced and the DNA synthesis decreased (p<0.01). This enhancement augmented with the increasing doses of verapamil (p<0.01). Although the initial stimulatory effect of verapamil on the DNA synthesis may have some role in the enhancement of etoposide cytotoxicity, the involvement of some other mechanisms is postulated.