Effects of GroBiotic®-A supplementation on growth performance, body composition and liver and intestine histological changes in European Seabass (Dicentrarchus labrax) juveniles

Yazıcı M., Mazlum Y., Naz M., Sayin S., Ürkü Atanasov Ç., Akaylı T.

SU URUNLERI DERGISI, vol.37, no.4, pp.389-396, 2020 (ESCI)

  • Publication Type: Article / Article
  • Volume: 37 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.12714/egejfas.37.4.10
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.389-396
  • Istanbul University Affiliated: Yes


The effects of GroBiotic®-A supplementation on growth performance, body composition, liver and intestine histology in European seabass (Dicentrarchus labrax) juveniles were evaluated. The commercial GroBiotic®-A was added to diets at four different levels (0, 1, 2 and 3%), three replicates and fed 4 times a day (9:00, 11:30, 14:00, 16: 30 hours) for 60 days as ad libitum. Total 480 European seabass juveniles with a starting weight of 1.40±0.08 g were randomly stocked into 12 tanks with a volume of 1 m3 . At the end of the study, the changes observed in weight, feed conversion ratios (FCR) and survival rates were calculated as 6.69 ± 5.35-7.40 ± 5.47, 0.80 ± 0.18-0.88 ± 0.20 and 96.6 ± 1.51-100 ± 0.0, respectively. When the body composition of the control and treatment groups were compared, no statistically significant differences were observed between the protein and lipid values (p>0.05), except ash (p <0.05). Histological sections of intestinal tissue; the number of goblet cells was higher than that of the control group. The highest values were determined in the group supplemented 2% GroBiotic®-A. The highest microvillus length was found in the group added 1% GroBiotic®-A. It was detected an inverse relationship between microvillus length and contribution rates as the the GroBiotic®-A additive levels increased. Also, degeneration and necrosis was detected in hepatocyte cells of seabass juvenile fed with diets supplemented 2% and 3% GroBiotic®-A as well as increase of the number of fatty vacuoles in liver tissue due to the increase in the amount of GroBiotic®-A. In conclusion, when the growth parameters, body composition and histological data were evaluated together, the feeding group supplemented 1% GroBiotic®-A performed the best.