Benomyl induced oxidative stress related DNA damage and apoptosis in H9c2 cardiomyoblast cells

Mehtap K., Ezgi O., Tugce B., Fatma K. E., Gul O.

TOXICOLOGY IN VITRO, cilt.75, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.tiv.2021.105180
  • Dergi Adı: TOXICOLOGY IN VITRO
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Apoptosis, Benomyl, Cardiotoxicity, H9c2 cells, Oxidative stress, NF-KAPPA-B, BENZIMIDAZOLE FUNGICIDE, ALDEHYDE DEHYDROGENASE, IN-VITRO, CARBENDAZIM, JNK, AROMATASE, DYNAMICS, CARDIOTOXICITY, PATHOGENESIS
  • İstanbul Üniversitesi Adresli: Evet

Özet

Benomyl, benzimidazole group pesticide, has been prohibited in Europe and USA since 2003 due to its toxic effects and it has been still determined as food and environmental contaminant. In the present study, the toxic effect mechanisms of benomyl were evaluated in rat cardiomyoblast (H9c2) cells. Cytotoxicity was determined by MTT and NRU assay and, oxidative stress potential was evaluated by reactive oxygen species (ROS) production and glutathione levels. DNA damage was assessed by alkaline comet assay. Relative expressions of apoptosis related genes were evaluated; furthermore, NF-kappa B and JNK protein levels were determined. At 4 mu M concentration (at which cell viability was >70%), benomyl increased 2-fold of ROS production level and 2-fold of apoptosis as well as DNA damage. Benomyl down-regulated miR21, TNF-alpha and Akt1 > 48.75 and > 97.90; respectively. PTEN, JNK and NF-KB expressions were upregulated. The dramatic changes in JNK and NF-kappa B expression levels were not observed in protein levels. These findings showed the oxidative stress related DNA damage and apoptosis in cardiomyoblast cells exposed to benomyl. However, further mechanistic and in vivo studies are needed to understand the cardiotoxic effects of benomyl and benzimidazol fungucides.