IMMUNOHISTOCHEMICAL COMPARISON OF iNOS IN PERICORONAL DENTAL FOLLICLES AND PERIAPICAL INFLAMMATORY LESIONS


Kocaelli H. , Keklikoglu N.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, vol.23, no.3, pp.1383-1387, 2009 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 3
  • Publication Date: 2009
  • Doi Number: 10.1080/13102818.2009.10817675
  • Journal Name: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1383-1387
  • Keywords: asymptomatic impacted third molar, dental follicle, inducible nitric oxide synthase, nitric oxide, periapical granuloma, radicular cyst, NITRIC-OXIDE SYNTHASE, GAMMA-PRODUCING CELLS, HEAT-SHOCK PROTEINS, RADICULAR CYSTS, 3RD MOLARS, EXPRESSION, TISSUES

Abstract

Nitric oxide, produced by inducible nitric oxide synthase (iNOS), has been implicated in the pathogenesis of periapical inflammatory, lesions (PILs); however, its existence in pericoronal dental follicles (DFs) of asymptomatic unerupted third molars is not entirely clear. The aim of this study is to analyze and compare the presence of iNOS immunoreactivity (iNOS-IR) in periapical granulomas (PGs) and radicular cysts (RCs), which are two different stages of PILs in chronic inflammatory process, and in DFs which may transform into chronic inflammatory tissues, cysts and other pathologies. In this study, immunohistochemical method was performed for evaluation of PGs (n=20), RCs (n=33) and DFs (n=24) groups from formalin-fixed, paraffin-embedded sections, in terms of iNOS-IR. The statistical analyses showed that there were no significant differences between PGs, RCs and DFs groups regarding iNOS-IR (p>0.05). The results of this study suggest that the extraction of asymptomatic impacted third molars with DFs is necessary for prophylactic purpose.