10. Uluslararası Bilimsel Araştırmalar Kongresi, Ankara, Turkey, 10 - 11 April 2021, pp.11-18
Many species are belonging to the genus Centaurea, which the rate of endemism 61% in Turkey, used to treat
many diseases traditionally among the people and thus the increasing bioactivity studies related to these species.
Different extracts from Centaurea zaferii Negaresh plant used in this study were found to be rich in total phenolic
(TPC) and flavonoid content (TFC), and the extracts were found to be strong in both in-vitro antioxidant tests and
inhibition of diabetes-associated enzymes (α-amylase and α-glucosidase). It was determined that TPC (21,76 mg
GAE/g) was higher in methanol extract, while in dichloromethane extract, TFC (489.74 mg RE/g) was higher, obtained
by maceration. It was determined that dichloromethane extract was more effective in phosphomolybdenum (164.55
mg TE/g) antioxidant test and α-amylase (1073,71 mg ACE/g) inhibition, methanol extract in DPPH (6.688 mg TE/g),
ABTS (33.28 mg TE/g), CUPRAC (61,71 mg TE/g), FRAP (82,22 mg TE/g) antioxidant tests, and α-glucosidase
(1147.00 mg ACE/g) inhibition. As a result, further phytochemical studies are planned to determine the active
compounds in C. zaferii Negaresh plant extracts' content and determine the different biological activities of these
extracts besides enzyme inhibition and to be published in an international journal.