Akademik Veri Yönetim Sistemi
III. International Agricultural, Biological & Life Science Conference, Edirne, Türkiye, 29 - 31 Ağustos 2022, ss.536
Carrot (Daucus carota), a member of the Apiaceae family, is rich in antioxidants. Especially
purple carrot plants produce a secondary metabolite from the flavonoid family called
anthocyanin. These secondary metabolites are known to have significant antioxidant activity.
Salinity is an abiotic stress that threatens crop yield, including disturbing osmotic equilibrium
and generating reactive oxygen species in plant cells. Exposure to salinity is known to induce
or stimulate the production of plant seconder metabolites. Salicylic acid (SA, 2-hydroxybenzoic
acid), one of the plant growth regulators, is used in the stimulation of many signaling pathways
involved in various plant defense responses. The aim of this study is application of different
elicitors to increase anthocyanin accumulation by activation of plantdefense pathways. In this
regard; seeds of ''Daucus carota ssp. (purple carrot)'' taken from the ‘’Republic of Türkiye
Ministry of Agriculture and Forestry Atatürk Horticultural Central Research Institute’’ were
used and studies were carried out to obtain callus culture. First of all, the seeds were surface-
sterilized and MS medium was prepared for seeds to plant and germinate. Explants which were
obtained from hypocotyl of germinating plants were used to establish callus cultures in MS
medium containing 50 uL of BAP (6-Benzylaminopurine) and 500 uL of 2,4-D (2,4-
dichlorophenoxyacetic acid). 1 month-old calli were exposed to different concentrations of
saliyclic acid and sodium chloride, then the changes in anthocyanin amounts and growth ratios
were calculated through 20 days and samples were taken in every 5 days. Anthocyanin amounts
in the calli were analyzed by UV-visible spectrophotometer method and fresh weights were
measured. Results of growth measurements has showed that, when growth rate of control group
was increased 5.7 fold at the end of 20 days, in 50 mM NaCl it was 6.04 fold and 3.6 fold in 50
uM SA, respectively. The outcomes of this study might serve to understand better the
relationship between the anthocyanin production and salicylic acid, salinity.