Investigation of plasmid-mediated quinolone resistance among isolates obtained in a Turkish intensive care unit

Nazik H., Ongen B., Kuvat N.

JAPANESE JOURNAL OF INFECTIOUS DISEASES, cilt.61, sa.4, ss.310-312, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 61 Sayı: 4
  • Basım Tarihi: 2008
  • Dergi Adı: JAPANESE JOURNAL OF INFECTIOUS DISEASES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.310-312
  • İstanbul Üniversitesi Adresli: Evet

Özet

The aim of this study was to search for three plasmid-encoded, quinolone-resistance determinants: qnrA, qnrB, and qnrS. Thus, 460 Gram-negative strains belonging to 11 different genera (clinical, 347; non-clinical, i.e., from a rectal swab, 113), of which 40% were ciprofloxacin-resistant, were recovered from patients in an intensive care unit at the Istanbul Medical Faculty, Turkey, in the years 2000 and 2006. PCR with primers specific for qnrA, qnrB, and qnrS genes and primers specific for a series of ESBL genes were used. One qnrB1 and two qnrS1 genes were identified in three ESBL-positive isolates, whereas no qnrA-positive strain was found. The qnrB1 determinant was identified in a ciprofloxacin-susceptible Enterobacter cloacae isolate that expressed CTX-M-15 beta-lactamase. Two qnrS1-determinants were found in two ciprofloxacin-susceptible E. cloacae isolates that were clonally related, but that had been isolated from different patients; both of these isolates expressed the same ESBL, CTX-M-3. This study detected the first plasmid-mediated quinolone-resistance determinants qnrB1 and qnrS1, among clinical strains obtained from patients in Turkey.

Investigation of plasmid-mediated quinolone resistance among isolates obtained in a Turkish intensive care unit

By:Nazik, H (Nazik, Hasan)[ 1 ] ; Ongen, B (Ongen, Betigul)[ 1 ] ; Kuvat, N (Kuvat, Nuray)[ 1 ]

 

JAPANESE JOURNAL OF INFECTIOUS DISEASES

 

Volume: 61

 

Issue: 4

 

Pages: 310-312

Published: JUL 2008

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Abstract

The aim of this study was to search for three plasmid-encoded, quinolone-resistance determinants: qnrA, qnrB, and qnrS. Thus, 460 Gram-negative strains belonging to 11 different genera (clinical, 347; non-clinical, i.e., from a rectal swab, 113), of which 40% were ciprofloxacin-resistant, were recovered from patients in an intensive care unit at the Istanbul Medical Faculty, Turkey, in the years 2000 and 2006. PCR with primers specific for qnrA, qnrB, and qnrS genes and primers specific for a series of ESBL genes were used. One qnrB1 and two qnrS1 genes were identified in three ESBL-positive isolates, whereas no qnrA-positive strain was found. The qnrB1 determinant was identified in a ciprofloxacin-susceptible Enterobacter cloacae isolate that expressed CTX-M-15 beta-lactamase. Two qnrS1-determinants were found in two ciprofloxacin-susceptible E. cloacae isolates that were clonally related, but that had been isolated from different patients; both of these isolates expressed the same ESBL, CTX-M-3. This study detected the first plasmid-mediated quinolone-resistance determinants qnrB1 and qnrS1, among clinical strains obtained from patients in Turkey.