Evaluation of the cytotoxic effect of Ly2109761 on HeLa cells using the xCELLigence RTCA system


Cetin I., Topcul M. R.

ONCOLOGY LETTERS, vol.17, pp.683-687, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17
  • Publication Date: 2019
  • Doi Number: 10.3892/ol.2018.9556
  • Journal Name: ONCOLOGY LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.683-687
  • Keywords: transforming growth factor- receptor inhibitor, HeLa cell culture, xCELLigence Real-Time Cell Analysis DP instrument, GROWTH-FACTOR-BETA, CANCER, INHIBITOR
  • Istanbul University Affiliated: Yes

Abstract

In the present study, the in vitro cytotoxic effect of a novel transforming growth factor- receptor inhibitor, LY2109761, was investigated in the human cervix carcinoma HeLa cell lines. For the purpose of the present study, cell index values obtained using the xCELLigence Real-Time Cell Analysis DP instrument, and mitotic, labelling and apoptotic index analysis were used. The results of the present study indicated that LY2109761 affected the cytoskeleton of HeLa cells, decreased the mitotic and labelling index values of the HeLa cell line, and increased the apoptotic index values. Significant differences were observed between the control group which was not treated with LY2109761 and the experimental groups, which were treated with LY2109761 (P<0.01). The results of the present study suggest that LY2109761 may serve as a promising treatment option for cervix carcinoma.