Akademik Veri Yönetim Sistemi
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.28, sa.2, ss.193-200, 2022 (SCI-Expanded)
Abstract: Usage of oocytes obtained from ovaries aft er long-term cold storage for in vitro embryo production is a promising tool for the protection
of wildlife and endangered animal species. Mammalian oocytes are susceptible to oxidative stress with regard to the high lipid content of plasma
membranes. Melatonin is known as a powerful antioxidant and anti-apoptotic agent due to its ability to eliminate toxic oxygen derivatives and
reduce the formation of reactive species. Th is study was performed to verify the optimal environmental conditions for long-term preservation of
cat ovaries (Felis domesticus) by adding diff erent concentrations of melatonin (500, 750 and 1000 µM) to the storage medium (0.9% NaCl) as an
antioxidant to be preserved at 4°C for 24 h. To determine the eff ect of melatonin on cat oocytes collected from stored ovaries, the anti and proapoptotic gene levels in cumulus oophorus, the in vitro maturation rates, the cell membrane and oocytes viability were evaluated. In all melatonin
added groups regardless of whether they are stored in the cold; Pro-apoptotic gene levels (BAX) were determined to be upregulated however, antiapoptotic gene levels (BCL-2) were downregulated in cumulus cells (P<0.05). Th e cell membrane stability and cell viability rates of oocytes began
to deteriorate in parallel with the rate of melatonin increase. In parallel with these findings, in vitro maturation rates of oocytes were negatively
aff ected as the amount of melatonin increased (P≤0.001). In conclusion the results showed that adding melatonin (500,750 or 1000 µM) to the
ovarian transport and storage medium had negative eff ect on in vitro maturation rate, viability and cell membrane structure of cat oocytes.