Biosecurity (BS) is a multidimensional preventive medicine approach that has health, technical, and financial aspects. The purpose of this study was to determine the differences of the BS scores belonging to the livestock enterprises depending on the geographical region and breeding types and to predict the socioeconomic factors having an impact over the BS level. The study was conducted with 517 breeders who live in 7 different regions of Turkey. The results have revealed that the enterprises performing "beef cattle fattening and dairy cattle breeding together" have higher BS scores than the enterprises in the other breeding category (P < 0.01). The differences between the technical and financial scores according to geographical regions were statistically significant (P < 0.01). The regions categorized as "west" have the highest score and "south" have the lowest score. It was determined that as the "educational level of the breeder" and "enterprise scale" increased, the BS score significantly went up. The regression model was found significant as a whole (P < 0.01) and the independent variables explained the variation in the technical and financial BS scores to be 14.6% and 12.7%, respectively. Dissemination of education/training practices and increasing enterprise scales will positively affect the level of BS in the sector.