A Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases


Inci F., Ozen M. O. , Saylan Y., Miansari M., Cimen D., Dhara R., ...Daha Fazla

ADVANCED SCIENCE, cilt.5, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 5 Konu: 9
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/advs.201800121
  • Dergi Adı: ADVANCED SCIENCE

Özet

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis(X)), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epithelial fraction. Captured sperm are then lysed on-chip and sperm DNA is isolated. This method reduces assay-time from 8 h to 80 min, providing an inexpensive alternative to current differential extraction techniques, accelerating identification of suspects and advancing public safety.