There are several in vivo systems constructed by generating reciprocal translocation between engineered chromosomal loci of yeast, but not without previous genome modifications. The experimental possibility of inducing chromosomal translocations between any two desired genetic loci in a eukaryotic model system will be instrumental in elucidating the molecular mechanism underlying genome re-organizations formed by DNA integration. DNA is able to undertake many different structures other than the familiar B-DNA helix. One of the important examples is the left-handed Z-DNA confermer. The biological role of Z-DNA is an area of active study. Z-DNA formation at a promoter has been shown to correlate with transcriptional activity. Also Z-DNA often behaves as an enhancer of gene expression. In this study, we reported that BIT driven by Z-DNA formations can be defined using web-based servers such as non-B DB v2.0 and SIDD/Z-DNA in Saccharomyces cerevisiae. The application of web-based server approaches may ultimately allow scientists to view their own genomic feature annotations in the different DNA motifs and to aid in the formation of hypotheses regarding the potential role of these structures in other cellular processes.