Differences in responsivity of original cupric reducing antioxidant capacity and cupric-bathocuproine sulfonate assays to antioxidant compounds
ANALYTICAL BIOCHEMISTRY, cilt.423, sa.1, ss.36-38, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 423 Sayı: 1
- Basım Tarihi: 2012
- Doi Numarası: 10.1016/j.ab.2012.01.011
- Dergi Adı: ANALYTICAL BIOCHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.36-38
- İstanbul Üniversitesi Adresli: Evet
Özet
The cupric reducing antioxidant capacity (CUPRAC) method using bathocuproine sulfonate (BCS) as cuprous-chelating ligand claims to be an efficient substitute for the conventional CUPRAC assay using neocuproine; however, differences in their physical chemistry give rise to significantly varying responsivities to antioxidant compounds. The antioxidant capacity due to beta-carotene (in acetone or dichloromethane) was fully measurable by conventional CUPRAC but not at all by cupric-BCS assay. Furthermore, CUPRAC exhibits distinctly stronger responses than cupric-BCS to naringenin, alpha-tocopherol, and bilirubin. (C) 2012 Elsevier Inc. All rights reserved.