Differential Pulse Voltammetric Determination of Anticancer Drug Regorafenib at a Carbon Paste Electrode: Electrochemical Study and Density Functional Theory Computations
JOURNAL OF ANALYTICAL CHEMISTRY, vol.75, pp.691-700, 2020 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 75
- Publication Date: 2020
- Doi Number: 10.1134/s1061934820050032
- Journal Name: JOURNAL OF ANALYTICAL CHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Biotechnology Research Abstracts, Chimica, Compendex, Food Science & Technology Abstracts
- Page Numbers: pp.691-700
- Keywords: regorafenib, voltammetry, determination, plasma, urine, density functional theory, MULTIKINASE INHIBITOR REGORAFENIB, LIQUID-CHROMATOGRAPHY, HUMAN PLASMA, SIMULTANEOUS QUANTIFICATION, KINASE INHIBITORS, BAY 73-4506, METABOLITES, ASSAY, ENERGIES
- Istanbul University Affiliated: Yes
Abstract
Electrochemical properties of regorafenib (REG) were studied in 0.1 M Britton-Robinson buffer-methanol solutions (3 : 2, v/v) with pH between 3 and 8 at carbon paste electrode by cyclic and differential pulse voltammetry. The results exhibited irreversible anodic peak at about 0.90 V vs. Ag/AgCl, NaCl (3 M). The anodic peak was found to be diffusion-adsorption controlled. Mechanism of REG electrochemical reaction was studied by performing density functional theory computations and mass spectrometric analysis. A validated differential pulse voltammetry (DPV) technique for REG determination was performed. The calibration curve of REG on carbon paste electrode was linear in the concentration range of 0.5-13 mu g/mL and limit of detection was 0.10 mu g/mL. The recommended DPV method was used to detect REG in spiked plasma and urine specimens and average recoveries were 94%.