Immune response to 17 beta-estradiol involved in polymer gels: Antigen specificity and affinity of hybridoma clones


Basalp A., Mustafaeva Z., Mustafaev M., Bermek E.

HYBRIDOMA, vol.19, no.6, pp.495-499, 2000 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 6
  • Publication Date: 2000
  • Doi Number: 10.1089/027245700750053995
  • Journal Name: HYBRIDOMA
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.495-499

Abstract

The immunogenic properties of 17 beta -estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17 beta -estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with K-d values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.