Akademik Veri Yönetim Sistemi
Biology Bulletin, cilt.52, sa.15, ss.1-18, 2025 (Scopus)
The genus Aeromonas, specifically the species A. hydrophila (Sawyer, 1986) and A. sobria (McCovan and Steinberg, 1995), has been identified for the first time in the Western Black Sea [BK] and Manyas Lake [M] through species identification using the API 20E test system and metagenomic analysis. This study presents a metagenomic analysis of bacterial isolates sourced from various locations of Küçükçekmece Lake, Manyas Lake, and the Western Black Sea Region. The research was conducted at the Istanbul University Department of Biology, Department of Basic and Industrial Microbiology (TEMA) Laboratory. The isolate was subjected to phenotypic analysis using fundamental biochemical methods, which included conventional biochemical tests and two commercially available systems, Vitek (GNI+) and API (20E). Genotypically, it was compared through multi-array alignment based on the 16S bacterial rRNA gene. By applying the polymerase chain reaction (PCR) and sequencing the 16S ribosomal RNA (rRNA) gene, the isolated bacterial genomes were amplified and sequenced. Following repeated sequence alignment and position correction, the resultant sequences were compared to known 16S rRNA gene sequences in the SILVA (Version 99) database.