Effect of exogenous melatonin on ethanol-induced changes in Na+,K+- and Ca2+-ATPase activities in rat synaptosomes


Oner P., Cinar F., Kocak H., Gurdol F.

NEUROCHEMICAL RESEARCH, cilt.27, ss.1619-1623, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 27 Konu: 12
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1023/a:1021678809464
  • Dergi Adı: NEUROCHEMICAL RESEARCH
  • Sayfa Sayıları: ss.1619-1623

Özet

In the present study, the effects of acute ethanol ( EtOH) toxicity and of exogenous melatonin (MLT) on this toxicity were examined by measuring membrane-bound ATPases and acetylcholinesterase activities in rat synaptosomal membranes. The concentrations of plasma alpha-tocopherol and adrenal ascorbic acid (AA) were also measured. Synaptosomal Na+, K+- ATPase and Ca2+-ATPase activities were significantly depressed in acute EtOH-intoxicated rats compared with controls, while acetylcholinesterase activity remained unaltered. Pretreatment with MLT (10 mg/kg) prior to acute EtOH administration prevented EtOH-induced inhibition of ATPases. Adrenal AA and plasma alpha-tocopherol levels were also depressed regardless of MLT pretreatment. MLT treatment alone affected neither membrane-bound enzyme activities nor tissue and blood levels of vitamins C and E. It is concluded that acute EtOH intoxication disturbs neural transport functions through the membrane-bound ATPase activity depression. Reduced AA and alpha-tocopherol levels may contribute to the neurodegenerative effects of EtOH. However, pretreatment with a high dose of MLT before EtOH administration may be beneficial to prevent EtOH-induced toxicity on ATPase-mediated neural transport functions.