Rapid isocratic HPLC investigation of radiochemical purity for Y-90-DOTATATE
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, vol.289, no.2, pp.573-575, 2011 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 289 Issue: 2
- Publication Date: 2011
- Doi Number: 10.1007/s10967-011-1119-6
- Journal Name: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.573-575
- Keywords: Yttrium-90, DOTA-peptides, DOTA-TATE, Somatostatin analogs, HPLC, RECEPTOR-POSITIVE TUMORS, RADIONUCLIDE THERAPY, NEUROENDOCRINE TUMORS, SOMATOSTATIN ANALOG, DOTA-PEPTIDES, Y-90, BIODISTRIBUTION, OCTREOTIDE, BINDING, IN-111
- Istanbul University Affiliated: No
Abstract
DOTA-conjugated peptides, such as [DOTA(0), Tyr(3)]ocreotide (DOTATOC) and [DOTA(0), Tyr(3)]octreotate (DOTATATE) can be labeled with radionuclides such as Y-90, Lu-177 and In-111 at high specific activities. These radiolabelled somatostatin analogues are used for peptide receptor radionuclide therapy (PRRT). Currently, radio-high performance liquid chromatography (radio-HPLC) and radio-thin layer chromatography (radio-TLC) are the methods of choice for the analysis of the labeled compounds. In literature, radiochemical purity of the radiolabelled DOTATATE was investigated using gradient reversed-phase radio-HPLC. However, these studies indicate long retention time of the radiolabelled compound of 14.52 min. In our study, a new simple and rapid reversed-phase isocratic system enables the radiochemical purity of the radiolabelled DOTATATE within a few minutes.