Rapid isocratic HPLC investigation of radiochemical purity for Y-90-DOTATATE

Tasdelen B., Ergun A., BÜYÜKKAYA F.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.289, sa.2, ss.573-575, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 289 Sayı: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s10967-011-1119-6
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.573-575
  • Anahtar Kelimeler: Yttrium-90, DOTA-peptides, DOTA-TATE, Somatostatin analogs, HPLC, RECEPTOR-POSITIVE TUMORS, RADIONUCLIDE THERAPY, NEUROENDOCRINE TUMORS, SOMATOSTATIN ANALOG, DOTA-PEPTIDES, Y-90, BIODISTRIBUTION, OCTREOTIDE, BINDING, IN-111
  • İstanbul Üniversitesi Adresli: Hayır

Özet

DOTA-conjugated peptides, such as [DOTA(0), Tyr(3)]ocreotide (DOTATOC) and [DOTA(0), Tyr(3)]octreotate (DOTATATE) can be labeled with radionuclides such as Y-90, Lu-177 and In-111 at high specific activities. These radiolabelled somatostatin analogues are used for peptide receptor radionuclide therapy (PRRT). Currently, radio-high performance liquid chromatography (radio-HPLC) and radio-thin layer chromatography (radio-TLC) are the methods of choice for the analysis of the labeled compounds. In literature, radiochemical purity of the radiolabelled DOTATATE was investigated using gradient reversed-phase radio-HPLC. However, these studies indicate long retention time of the radiolabelled compound of 14.52 min. In our study, a new simple and rapid reversed-phase isocratic system enables the radiochemical purity of the radiolabelled DOTATATE within a few minutes.